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Academic Degrees
Professional Experience
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Assistant
Professor -
UNC Charlotte, Dept. of Biology, 07/2006-present
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Research Assistant Professor
- University of Florida College of Medicine,
Dept. of Molecular Genetics and Microbiology, [Mentor: Dr.
Sue A. Moyer] 2004-2006
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Postdoctoral Research
Associate - University
of Wisconsin-Madison, Institute for Molecular
Virology,
[Mentor: Dr. Paul G. Ahlquist] 2001 – 2004
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Postdoctoral Research
Associate - University
of Florida, Citrus Research and Educational Center, [Mentor: Dr. William O. Dawson] 1999-2000
Research Interests
The major objective of my research is
to understand the mechanisms of viral RNA
replication and transcription in the nonsegmented
negative-strand RNA viruses (the order
Mononegavirales) represented by a wide variety
of human, animal (vertebrates as well as
invertebrates) and plant viruses, where the RNA
polymerase complex possesses unique features. The
order Mononegavirales includes many important
human and animal pathogens, such as rabies,
measles, Ebola, Marburg,
Nipah, Hendra, Borna disease, parainfluenza, Newcastle disease,
rinderpest, and other viruses. Importantly,
the Ebola and Marburg viruses are classified as
category A bioweapon agents and are currently
considered to be one of the bioterrorism threats. A
detailed understanding of the mode RNA synthesis in
these viruses is fundamental to
developing reagents to prevent and combat these
deadly diseases. To provide insights into the
biology of these viruses, my studies employ two
prototypic Mononegavirales, vesicular
stomatitis virus (VSV, a
rhabdovirus) and Sendai
virus (paramyxovirus), which serve as attractive
models due to a lack of serious pathogenicity for
humans and their very efficient replication in a
wide range of cell lines. The two-virus approach
allows the identification of universal as well as
virus-specific mechanisms of viral RNA
replication/transcription, because VSV and Sendai
virus belong to the different families within the
order Mononegavirales. Another direction of
my research will focus on the exploitation of VSV
and Sendai virus as clinically important vaccine and oncolytic vectors.
Vesicular stomatitis virus (VSV) life cycle
VSV Ebola virus Sendai virus
Courses Taught
Lab Members (left to right):
Amanda Sunderland, Valery Grdzelishvili, Nirav Shah,
Josh Stokell,
Megan Moerdyk-Schauwecker, Andrea Murphy
Important Links
Selected Publications
Furr S.R., Chauhan V.S., Sterka D.Jr.,
Grdzelishvili V.,
and Marriott I. 2008 “Characterization of
retinoic acid-inducible gene-I expression in primary
murine glia following exposure to vesicular
stomatitis virus”,
Journal
of Neurovirology, in press.
Grdzelishvili V.Z.,
Smallwood S., Tower D., Hall R.L., Hunt D.M., and
Moyer S.A. 2006
“Identification of a new region in the vesicular
stomatitis virus L protein which determines defects
in mRNA cap methylation”, Virology
350, 394-405
PDF
Grdzelishvili V.Z.,
Smallwood S., Tower D., Hall R.L., Hunt D.M., and
Moyer S.A. 2005 “A
single amino acid change in the L polymerase protein
of vesicular stomatitis virus completely abolishes
viral mRNA cap methylation”, Journal of
Virology 79, 7327-7337
PDF
Grdzelishvili V.Z., Garcia-Ruiz H., Watanabe
T., and Ahlquist P. 2005 “Mutual interference
between genomic RNA replication and subgenomic RNA
transcription in brome mosaic virus”,
Journal
of Virology
79,
1438-1451
PDF
Kushner D.B, Lindenbach B.D.,
Grdzelishvili V.Z., Noueiry A.O., Paul S.M., and
Ahlquist P. 2003 “Systematic, genome-wide
identification of host genes affecting replication
of a positive-strand RNA virus”, Proc. Natl.
Acad. Sci. USA 100, 15764-15769
PDF
Grdzelishvili V.Z.,
Chapman S.N., Dawson W.O. and Lewandowski D.J. 2000
“Mapping of the tobacco mosaic virus movement
protein and coat protein subgenomic RNA promoters
in vivo”, Virology 275, 177-192
PDF
Solovyev, A. G., Savenkov
E. I., Grdzelishvili V. Z., Kalinina N. O., Morozov S. Y., Schiemann J., and Atabekov J. G. 1999
“Movement of hordeivirus hybrids with exchanges in
the triple gene block”, Virology
253, 278-287
PDF
Solovyev, A. G., Zelenina
D. A., Savenkov E. I., Grdzelishvili V. Z.,
Morozov S. Y., Maiss E., Casper R., and Atabekov J.
G.. 1997 “Host-controlled cell-to-cell movement of a
hybrid barley stripe mosaic virus expressing a
dianthovirus movement protein”, Intervirology
40,1-6
Solovyev, A. G., Zelenina
D. A., Savenkov E. I., Grdzelishvili V. Z.,
Morozov S. Y., Lesemann D. E., Maiss E., Casper R.,
and Atabekov J. G.. 1996 “Movement of a barley
stripe mosaic virus chimera with a tobacco mosaic
virus movement protein”, Virology
217, 435-441. PDF
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