Research Interests

• Carolinas Medical Center, Charlotte, NC., 2007 – present. “Information Integration and Processing for Computer Aided Trauma Decision Making”. National Science Foundation, $450,000. Co-Principal Investigator:  Toan Huynh, MD. Principal Investigator:  Kayvan Najarian, PhD.
• Carolinas Medical Center, Charlotte, NC., 2003 - present. “Hepatic Microcirculatory Dysfunction after Trauma and Sepsis” The “AAST John H. Davis Scholarship Award”, $40,000.  Principal Investigator:  Toan Huynh, MD.
• Carolinas Medical Center, Charlotte, NC.  2003 - present. “Modulation of Hepatic Endothelial Dysfunction by Activated Protein C after Trauma and Sepsis” Lilly Corporation, $45,760. Principal Investigator:  Toan Huynh, MD.
• University of North Carolina, Charlotte, NC. 2001-present. “Consequences of Pneumoperitoneum-Induced Hepatic Blood Flow Reduction in Rats” Carolinas Laparoscopic and Advanced Surgery Program, $14,256. Co-Principal Investigator:  Toan Huynh, MD. Co-Principal Investigator:  Mark Clemens, PhD.
• University of North Carolina, Charlotte, NC. 2000-present.  “Sequential Injuries of Trauma and Sepsis: Kupffer Cell Mediated Liver Microcirculatory Dysfunction in Rats”Health Services Foundation (Grant #0200-764), $20,140.          Co-Principal Investigator:  Toan Huynh, MD. Co-Principal Investigator:  Mark Clemens, PhD.
• University of North Carolina, Charlotte, NC. 1999-present.“Effects of Trauma and Sepsis on Hepatic Microcirculation” Health Services Foundation (Grant #0200-581), $20,000. Co-Principal Investigator:  Toan Huynh, MD. Co-Principal Investigator:  Mark Clemens, PhD.
• Carolinas Medical Center, Charlotte, NC. 1999-present. “Mechanisms of Kupffer Cell Alterations after Trauma and Sepsis” Health Services Foundation (Grant #019902), $60,000. Principal Investigator: Toan Huynh, MD.
• University of North Carolina, Chapel Hill, NC. 1993-1995. “Mechanisms of Kupffer Cell Alterations after Trauma and Sepsis” NIH-NRSA, Trauma Training Grant (T32-GM08450). Advisors:  Christopher C. Baker, MD; Dept. of Surgery John J. Lemasters, MD Ph.D., Department of Cell Biology.
• University of Washington, Seattle, WA. 1988-1990.  “Intracellular Magnesium Fluxes in Response to Interleukin-1 and Gamma Interferon in the Murine pre-B 70Z/3 Cell Line” Medical School Thesis Advisor:  Karol Bomsztyk, MD; Dept. of Nephrology.
• University of Washington, Seattle, WA.  1984-1986.  “Interleukin-1: Mechanism of T-cell & B-cell Activation” B.S. Honors Thesis. Advisor:  Thomas Stanton, Ph.D.; Dept. of Immunology. “Motheaten: A Murine Model for Immunodeficiency and Autoimmune Diseases”. Undergraduate Honors Research Training Advisor:  Thomas Stanton, Ph.D.; Dept. of Immunology.

Professional/Academic Experience

• University of Washington, Seattle, Washington, Teaching Assistant, Anatomy - School of Medicine, 1995 - 1998
• University of North Carolina, Chapel Hill, North Carolina, Instructor, Adult Trauma Life Support Course, 1998 - 1999
• Carolinas Medical Center, Charlotte, North Carolina, Clinical Instructor, Department of General surgery, 1998 - present
• Carolinas Medical Center, Charlotte, North Carolina, Instructor, Advanced Trauma Life Support, 1999 - present
• Carolinas Medical Center, Charlotte, North Carolina, Instructor, Fundamental Critical Care Support, 2000 - present
• Carolinas Medical Center, Charlotte, North Carolina, Instructor, Advanced Cardiac Life Support, 1999 - present
• Carolinas Medical Center, Charlotte, North Carolina, Director, Surgical Intensive Care Unit, 1999 - present
• University of North Carolina, Chapel Hill, North Carolina, Clinical Assistant Professor of Surgery, 2000 - 2001
• University of North Carolina ,Charlotte, North Carolina, Adjunct Clinical Faculty, Graduate Program in Biology, 2001 - present
• University of North Carolina, Charlotte, North Carolina, Adjunct Associate Professor of Biology, 2001 - present
• Central Piedmont Community College, Charlotte, North Carolina, Medical Director, Department of Respiratory Therapy, 2002 - present
• Carolinas Medical Center, Charlotte, North Carolina, Director, Trauma Research.

Honors and Awards

Undergraduate: University of Washington

Graduated with Distinction in Microbiology-Immunology

Charles Evans Award: Top Departmental Graduate.  Magna Cum Laude

Golden Key National Honors Society 84-86

Office of the Dean-Honors Committee Certificate 84

Scholarships for Academic Excellence 82-86.

Medical School: University of Washington, Seattle

Chinese American Physicians’ Society Scholarship 1987

John & Mary Wilson; Dunlap Academic Scholarships 86-89

Postgraduate Residency: University of North Carolina, Chapel Hill.

First-Place Winner, Resident Paper Competition - 1994,

                   North Carolina Chapter - American College of Surgeons

First-Place Winner, Resident Paper Competition - 1994,

                   North Carolina Chapter - Committee on Trauma.

Second-Place Winner, Resident Paper Competition - 1994,

          Region IV, American College of Surgeon - Committee on Trauma.

Professional: Attending of the Year 2001, Department of General Surgery, Carolinas Medical Center, Charlotte, NC.

The AAST John H. Davis Research Scholarship Award 2003.

Fellowship, American College of Critical Care Medicine 2004.

Education

• University of Washington, Seattle, Washington, B.S. Degree, Microbiology-Immunology, 1986 - 1990

• University of Washington, Seattle, Washington, M.D. Degree, School of Medicine, 1990 - 1991

• University of North Carolina, Chapel Hill, North Carolina, Internship, General Surgery, 1991 - 1997

• University of North Carolina, Chapel Hill, North Carolina, Residency, General Surgery, 1997 - 1998

• University of North Carolina, Chapel Hill, North Carolina, Fellowship, Trauma/Critical Care.

Selected Publications

ABSTRACTS: 

1.         T Huynh, RT Currin, Y Tanaka, JJ Lemasters, CC Baker.  Activation of Kupffer Cells in vivo Following Femur Fracture.  Abstracts to the Fourteenth Annual Meeting of the Surgical Infection Society, 1994; Program book: p74. 

2.         T Huynh, RT Currin, Y Tanaka, CC Baker, JJ Lemasters.  Sustained Activation of Kupffer Cells in vivo Following Femur Fracture.  Gastroenterology 1994;106(4 Suppl):A703 

3.         T Huynh, L Bracey, JJ Lemasters, and CC Baker.  Effects of Femur Fracture on Kupffer Cell-induced Hepatic Oxygen Consumption and Lipopolysaccharide-stimulated Kupffer Cell Nitric Oxide Production.  The Thirty-seventh Annual University Surgical Residents’ Conference, the Society of University Surgeons, 1995; Program book. 

4.         T Huynh, LW Bracey, JJ Lemasters, CC Baker.  Kupffer Cell Activation after Trauma.  Toxicologist, 1995;15:313-314. 

5.         T Huynh, LW Bracey, JJ Lemasters, CC Baker.  Femur Fracture Trauma Induces Early Alterations in Kupffer Cell Proinflammatory Phenotype.  FASEB 1995; 9(3):A571(3312). 

6.         T Huynh, LW Bracey, JJ Lemasters, CC Baker.  Alterations in Kupffer Cell After Injury and Sepsis.  The 55^th Annual Meeting of the American Association for the Surgery of Trauma.  Program book, 1995. 

7.         T Huynh, LW Bracey, CC Baker, JJ Lemasters.  Kupffer Cell Pro-inflammatory Alterations after Trauma and Sepsis.  Hepatology, 1995;22(4 Suppl.):366A. 

8.         XX Peng, RT Currin, T Huynh, CC Baker, RG Thurman, and JJ Lemasters.  Femur Fracture Trauma to Donors Increases Graft Survival after Orthotopic Rat Liver Transplantation.  Hepatology, 1995;22(4 Suppl.):473A. 

9.         T Huynh, JS Guy, T Osler, R Rutledge.  An ICD-9 Based Illness Severity Score (ICISS) Outperforms APACHE II in Predicting Survival, Hospital Charges and Length of Stay in Surgical Intensive Care Unit Patients.  Critical Care Medicine 1998;26(Suppl):A25. 

10.     T Huynh, JS Guy, E Rutherford, R Rutledge.  Incidence and Outcome of Delayed Gastrointestinal Injury After Blunt Abdominal Trauma:  An Analysis of 569 Patients.  J Trauma, 1998;44:424. 

11.     T Huynh, R Rutledge, JS Guy, CC Baker.  Declining Rate of Common Bile Duct Operations:  A Statewide, Five-year, Time-series Analysis.  Gastroenterology 1998. 

12.     R Rutledge, T Huynh, JS Guy, EJ Rutherford.  Analysis of 25 Milliman and Robertson Guidelines for Surgery.  Abstracts to the 118^th Annual Meeting of the American Surgical Association.  Program book, 1998. 

13.     JS Guy, R Rutledge, MD Peck, T Huynh.  Discordance in Outcome between Burn and Non-burn Centers:  Incorrect Coding of Burn Size may Overestimate Non-burn Center Performance.  The Annual Meeting of the American Association for the Surgery of Trauma.  Program book, 1998. 

14.     JS Guy, EJ Rutherford, T Huynh, CC Baker, AA Meyer.  Right Ventricular End Diastolic Volume Index is Not Affected by Increases in Positive End Expiratory Pressure.  J Trauma, 1998;45:1117. 

15.     T Huynh, M Messer, WS Miles, DG Jacobs, RF Sing, MH Thomason.  Positive End Expiratory Pressure Alters Intracranial and Cerebral Perfusion Pressure in Severe Traumatic Brain Injury.  J Trauma, 1999;47:213 

16.     T Huynh, J Sarafin, DG Jacobs, W Miles, RF Sing, MH Thomason.  Short Delay in Enteral Nutrition Does Not Affect Outcome.  J Trauma 1999;47:1178. 

17.     RF Sing, NA Widder, J McMullen, JP Coyle, T Huynh.  Utilization of Respiratory Therapists for In-Hospital Endotracheal Intubation.  Crit Care Med 1999;27(Suppl.):A39. 

18.     RF Sing, JA Watts, T Huynh, BT Heniford, WJ Messick.  Paradoxic Venous Hypercarbia Occurs During Hemorrhagic Shock.  Shock 2000;13:(Suppl 1):142. 

19.     R Baveja, N Kresge, Y Yokoyama, JX Zhang, T Huynh, MG Clemens.  Hyporesponse of Hepatic Microcirculation to Endothelin in Sepsis.  FASEB 2000;14(4):A32. 

20.     R Baveja, N Kresge, Y Yokoyama, N Sonin, JX Zhang, T Huynh, MG Clemens.  Effects of Fluid Resuscitation on the Pattern of Vascular Gene Expression During Abdominal Sepsis.  SHOCK 2000;13:11. 

21.     R Baveja, N Kresge, Y Yokoyama, N Sonin, JX Zhang, MG Clemens, T Huynh.  Shunting of Hepatic Microcirculation After Polymicrobial Sepsis.  J Am Coll Surg 2000;191(4):S33. 

22.     T Huynh, J Sarafin, DG Jacobs, W Miles, RF Sing, MH Thomason.  Implementation of A Nutrition Guideline Improves Outcomes in Severely Injured Patients.  J Trauma 2000 (in press). 

23.     R Baveja, N Kresge, Y Yokoyama, N Sonin, JX Zhang, MG Clemens, T Huynh.  Low Volume Resuscitation Exacerbates Hepatic Vascular Stress Gene Dependent Microvascular Dysfunction in Sepsis.  J Surg Res 2000;93:373.

24.     G Adrales, T Huynh, B Broering, W Miles, RF Sing, DG Jacobs, MH Thomason.  A Thoracostomy Tube Guideline Improves Management Efficiency in Trauma Patients. J Trauma (in press). 

25.     G Mostafa, RF Sing, R McKeown, T Huynh, BT Heniford.  The Hazard of Scattered Radiation in a Trauma Intensive Care Unit.  Crit Care Med 2000;28(12S):A148. 

26.     R Baveja, J Ashburn, Y Yokoyama, N Sonin, JX Zhang, MG Clemens, T Huynh.  Remote Trauma Sensitizes Hepatic Microcirculation to Endothelin: A Potential Mechanism for Enhanced Injury in Sequential Stresses.  Surgical Infections 2001;2(1):79.

27.     R Baveja, J Ashburn, Y Yokoyama, JX Zhang, MG Clemens, T Huynh,.  Altered In Vivo Response of the Hepatic Microcirculation to Endothelin after Remote Trauma.  SHOCK 2001;15(S):66. 

28.     J Ashburn, R Baveja, Y Yokoyama, T Huynh, MG Clemens.  Overexpression of Caveolin-1: A Potential Mechanism for a Hyperresponse of Hepatic Microcirculation to Endothelin during Sepsis.  SHOCK 2001;15(S):65. 

29.     G Mostafa, T Huynh, RF Sing, WS Miles, MT Thomason.  Gender – Related Outcomes in Trauma Patients.  J Trauma 2001;51(6):1231. 

30.     SM Lee, K Korneszczuk, T Huynh, MG Clemens.  Mechanism of Hepatic Microvascular Dysfunction during Trauma and Sepsis.  SHOCK 2002;17(S):59. 

31.     S Keller, SM Lee, MG Clemens, T Huynh.  Ablation of Kupffer Cells Prior to Trauma and Sepsis Attenuates COX-2 Protein Expression.  J Surg Res 2002;107(2):276-277. 

32.  T Huynh, W Miles, M Scotton, T Nelson, G Dukes, RF Sing, M Thomason.  Efficacy of a Ventilator Weaning Program in Critically Injured Patients. Crit Care Med 2002;30(12S):A93. 

33.  S Keller, M Paxian, MG Clemens, T Huynh.  In vivo Assessment of Hepatic Microcirculation to Endothelin-1 after Trauma and Sepsis in Rats.  SHOCK 2003;19(S1):76. 

34.  S Keller, M Paxian, JH Ashburn, MG Clemens, T Huynh.  Alterations in Hepatic Microcirculation, Mitochondrial Redox State and Tissue Oxygenation after Trauma and Sepsis.  J Surg Res 2003;114(2):246-247. 

35.  J Burns, W Miles, T Huynh, et al.  High Frequency Oscillatory Ventilation Improves Oxygenation in Critically Ill Trauma and Surgical Patients with Acute Respiratory Distress Syndrome (ARDS).  Crit Care Med 2003;31(12S):A100. 

36.  W Miles, M Sturdevant, T Huynh, et al.  A Comparison of Drotrecogin Alfa (Activated) in Medical and Surgical Patients with Severe Sepsis.  Crit Care Med 2003;31(12S):A128. 

37.  S Keller, MG Clemens, T Huynh.  Role of Endothelin-B Receptor Subtypes on Hepatic Microcirculation after Trauma and Sepsis.  SHOCK 2004;21(S2):65 

38.  S Keller, JH Ashburn, MG Clemens, T Huynh.  Kupffer Cell Depletion Improves Hepatic Microcirculation and Redox State after Trauma and Sepsis.  J Trauma 2004;57: (in press) 

39.  J Burns, RF Sing, M Sturdevant, T Huynh, DG Jacobs, W Miles, MH Thomason.  The Role of Transesophageal Echocardiography in the Optimization of Fluid Resuscitation in the Critically Ill Patient.   J Trauma 2004:57:457. 

40.  W Miles, J Burns, T Huynh, MH Thomason, RF Sing, DG Jacobs, J Hylton, T Nelson. Operative Procedures May Be Safely Performed on Critically Ill Patients Receiving High Frequency Oscillatory Ventilation. Critical Care Med 2005;32(12S):A112. 

41.  J Hylton, T Pope, W Miles, J Burns, T Huynh. Successful Intra-hospital Transportation of Critically Ill Patients Requiring the 3100B High Frequency Oscillatory Ventilator. Critical Care Med 2005;32(12S):A112. 

42.  W Miles, J Hylton, J Burns, T Huynh, T Nelson, RF Sing, DG Jacobs, MH Thomason. High Frequency Oscillatory Ventilation Improves Oxygenation in Critically Ill Trauma Patients with Adult Respiratory Distress Syndrome. J Trauma 2005; (in press)

 REFEREED JOURNALS: 

1.         T Huynh, K Bomsztyk.  Effects of Interleukin-1 and gamma Interferon on Total Intracellular Magnesium Concentration in a Murine Lymphoid Cell Line.  Magnesium & Trace Elements 1992; 10(5-6):321-326. 

2.         T Huynh, RT Currin, Y Tanaka, JJ Lemasters, and CC Baker.  Activation of Kupffer Cells in vivo Following Femur Fracture.  Arch Surgery 1994; 129(12):1324-1329. 

3.         T Huynh, CC Baker, LW Bracey, and JJ Lemasters.  Adaptive Kupffer Cell Alterations after Femur Fracture Trauma in Rats.  Am J Physiol 1997; 272 (Gastrointest. Liver Physiol. 35):G1457-G1462. 

4.         JS Guy, EJ Rutherford, T Huynh, CC Baker, AA Meyer.  Right Ventricular End Diastolic Volume Index is Not Affected by Increases in Positive End Expiratory Pressure.  J Trauma, 1999;45:1117-1122. 

5.         T Huynh, LW Bracey, JJ Lemasters, and CC Baker.  Pro-inflammatory Kupffer Cell Alterations after Femur Fracture Trauma and Sepsis in Rats.  SHOCK 2000;14(5):555-560. 

6.         R Baveja, N Kresge, Y Yokoyama, N Sonin, JX Zhang, MG Clemens, T Huynh.  Shunting of Hepatic Microcirculation After Polymicrobial Sepsis.  Surg Forum 2000;22:62-65. 

7.         AD Perron, RF Sing, CC Branas, T Huynh.  Predicting Survival in Pediatric Trauma Patients Receiving CPR in the Prehospital Setting.  Prehosp Emerg Care 2001;5:6-9. 

8.         G Adrales, T Huynh, B Broering, W Miles, RF Sing, DG Jacobs, MH Thomason.  A Thoracostomy Tube Guideline Improves Management Efficiency in Trauma Patients. J Trauma 2002;52:210-216. 

9.         G Mostafa, RF Sing, R McKeown, T Huynh, BT Heniford.  The Hazard of Scattered Radiation in a Trauma Intensive Care Unit.  Crit Care Med 2002;30:574-576. 

10.     Y Yokoyama, DM Alterman, AH Sarmadi, R Baveja, JX Zhang, T Huynh, M Clemens.  Hepatic Vascular Response to Elevated Intraperitoneal Pressure in Rats.  J Surg Res 2002; 105:86-94. 

11.     T Huynh, M Messer, WS Miles, DG Jacobs, RF Sing, MH Thomason.  Positive End Expiratory Pressure Alters Intracranial and Cerebral Perfusion Pressure in Severe Traumatic Brain Injury.  J Trauma 2002;53:488-493. 

12.     G Mostafa, T Huynh, RF Sing, WS Miles, MT Thomason.  Gender – Related Outcomes in Trauma Patients.  J Trauma 2002;53:430-435. 

13.     R Baveja, N Kresge, JH Ashburn, S Keller, Y Yokoyama, N Sonin, JX Zhang, T Huynh, MG Clemens.  Potentiated Hepatic Microcirculatory Response to Endothelin-1 during Polymicrobial Sepsis.  Shock 2002;18(5):415-422. 

14.     M Paxian, SA Keller, T Huynh, MG Clemens.  Perflubron Emulsion Improves Hepatic Microvascular Integrity and Mitochondrial Redox State after Hemorrhagic Shock.  Shock 2003;20(5):449-457. 

15.     M Paxian, SA Keller, B Cross, T Huynh, MG Clemens.  High Resolution Visualization of Oxygen Distribution in the Liver Microcirculation in vivo.  Am J Physiol 2004;286:G37-G44. 

16.     JH Ashburn, R Baveja, N Kresge, K Korneszczuk, SA Keller, A Karaa, Y Yokoyama, JX Zhang, T Huynh, MG Clemens.  Remote Trauma Sensitizes Hepatic Microcirculation to Endothelin via Caveolin Inhibition of eNOS Activity.  SHOCK 2004;22(2):120-130. 

17.     M Paxian, SA Keller, R Baveja, K Korneszczuk, T Huynh, MG Clemens.  Functional Link between ETB Receptors and eNOS Maintain Tissue Oxygenation in the Normal Liver.  Microcirculation 2004;11(5):437-51. 

18.     S Keller, SM Lee, MG Clemens, T Huynh.  Ablation of Kupffer Cells Prior to Trauma and Sepsis Attenuates COX-2 Protein Expression.  J Surg Res 2005;124:126-133. 

19.     S Keller, M Paxian, JH Ashburn, MG Clemens, T Huynh.  Kupffer Cell Ablation Improves Hepatic Microcirculation after Trauma and Sepsis.  J Trauma; (in press). 

20.     J Burns, RF Sing, G Mostafa, T Huynh, DG Jacobs, W Miles, MH Thomason.  The Role of Transesophageal Echocardiography in Optimizing Resuscitation in Acutely Injured Patients.  J Trauma 2005; (in press). 

21.     O Gunter Jr., RF Sing, T Huynh. Minimally Invasive Management for Complications of Blunt Hepatic Trauma. Surgical Rounds 2005; (in press) 

22.     W Miles, J Hylton, J Burns, T Huynh, T Nelson, RF Sing, DG Jacobs, MH Thomason. High Frequency Oscillatory Ventilation Improves Oxygenation in Critically Ill Trauma Patients with Adult Respiratory Distress Syndrome. J Trauma 2005; (submitted). 

23.     T Huynh, J Sarafin, DG Jacobs, W Miles, RF Sing, MH Thomason.  Implementation of A Nutrition Practice Guideline Improves Outcome in Trauma Patients.  J Am Coll Surg; (manuscript in preparation). 

24.     RF Sing, NA Widder, J McMullen, JP Coyle, T Huynh.  Utilization of Respiratory Therapists for In-Hospital Endotracheal Intubation.  Chest; (manuscript in preparation). 

25.     R Baveja, J Ashburn, Y Yokoyama, N Sonin, JX Zhang, MG Clemens, T Huynh.  Remote Trauma Sensitizes Hepatic Microcirculation to Endothelin: A Potential Mechanism for Enhanced Injury in Sequential Stresses.  Am J Physiol (manuscript in revisions). 

26.     T Huynh, W Miles, M Scotton, T Nelson, G Dukes, RF Sing, M Thomason.  Efficacy of a Ventilator Weaning Program in Critically Injured Patients. Crit Care Med (manuscript in preparation). 

CASE REPORTS: 

1.         RF Sing, T Huynh, MA Gibbs, AD Perron.  The “Swallowed” Endotracheal Tube.  Am J Emerg Med 2001;19(7):606-607. 

BOOK CHAPTERS: 

1.         CC Baker and T Huynh.  Acute Pancreatitis-Surgical management.  In Critical Care Clinics.  R Fisher (ed).  WB Saunders, Philadelphia, PA.  1994, 11(2):311-322. 

2.         CC Baker and T Huynh.  Immunologic Response.  In Trauma (3rd Edition).  DV Feliciano, EE Moore and KL Mattox (eds.).  Appleton & Lange, Stamford, CT.  1995, pp1177-1191. 

3.         CC Baker and T Huynh.  Sepsis in the Critically Ill Patient.  In Current Problems in Surgery.  SA Wells (ed).  Mosby, St. Louis, MO.  1995, 32(12):1013-1092. 

4.         T. Huynh. The Comatose Patient.  In Trauma Decision Making – Initial Management of Injuries.  RF Sing, P Riley (eds.).  British Medical Association/BMJ Books, Tavistock Square, London.  2000, pp177-182. 

 LETTERS TO THE EDITORS: 

1.       RF Sing, MH Thomason, BT Heniford, WS Miles, T Huynh, DG Jacobs.  Venous Air Embolism from Central Venous Catheterization: Under-recognized or Over-diagnosed?.  Crit Care Med 2000;28(9):3377-3378.

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